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A PURIFICATION PROCESS FOR A CALCIUM BINDING TYPE ENZYME, SUCH AS A METALLOPROTEASE, USING A CALCIUM FREE BUFFER
A PURIFICATION PROCESS FOR A CALCIUM BINDING TYPE ENZYME, SUCH AS A METALLOPROTEASE, USING A CALCIUM FREE BUFFER
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机译:使用无钙缓冲剂的钙结合型酶(如金属蛋白酶)的纯化过程
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摘要
The present invention relates to an enzyme purification process. More particularly, the present invention relates to a protein purification process for calcium binding type enzyme. Specifically, in one aspect of the present invention there is provided a process for purifying a calcium binding type enzyme which is a mettalloprotease comprising: (a) preparing an unpurified solution which contains calcium binding type enzyme; (b) concentrating the solution; (c) diluting by adding calcium free buffer; (d) adjusting buffer concentration and pH, (e) incubating the concentrated solution to precipitate the protein; and (f) collecting the precipitate.
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