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A new DNA polymerase with proof-reading 3'-5' exonuclease activity
A new DNA polymerase with proof-reading 3'-5' exonuclease activity
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机译:具有校对3'-5'核酸外切酶活性的新型DNA聚合酶
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摘要
The invention relates to DNA polymerases which are capable of proofreading 3'-5' exonuclease activity during DNA sequencing of a DNA strand, such that the DNA polymerase functions to excise mismatched nucleotides from the 3' terminus of the DNA strand at a faster rate than the rate at which the DNA polymerase functions to remove nucleotides matched correctly with the nucleotides of the template, and which DNA polymerase does not exhibit 5'-3' exonuclease activity. The invention also relates to isolated and cloned DNA sequences derived from the Bacillus stearothermophilus thermostable DNA polymerase, as well as the expressed polymerase itself.
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