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Process for microinjection and nanopipette for introducing an injection product, particularly foreign genetic material in procaryotic or eucaryotic cells or cellcomparments thereof (plastides, cell nucleus)
Process for microinjection and nanopipette for introducing an injection product, particularly foreign genetic material in procaryotic or eucaryotic cells or cellcomparments thereof (plastides, cell nucleus)
A microinjection method (I) for introducing a material, especially foreign genetic material, into prokaryotic or eukaryotic cells and cell compartments (plastids and nuclei), using a nanopipette and pressure, is new. A microinjection method (I) for introducing a material, especially foreign genetic material, into prokaryotic or eukaryotic cells and cell compartments (plastids and nuclei), using a nanopipette and pressure, is new. The nanopipette has an outer diameter of 0.5 - 2mm, an inner diameter of 0.1 - 1.5mm and a point measurement of 0.025 - 0.3 mu m and is filled with the injection material mixed with a warmth-expanding material. The capillary of the nanopipette is then closed with an adhesive, the pipette point inserted into the plastid, bacterium, cell compartment or nucleus using a microscope and a micromanipulator and the nanopipette warmed using a heating apparatus. The injection material is passed into the plastid, bacterium, cell compartment or nucleus (which has a diameter of 1 - 20 mu l) with a speed of about 1 mu l per second. An Independent claim is also included for a nanopipette for performing (I).
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