An expression system for producing recombinant human erythropoietin, a method for purifying the secreted human erythropoietin and uses thereof

摘要

The present invention provides an expression system for producing recombinant human erythropoietin (rhEPO) exhibiting biological activity and immunochemical properties of the native human erythropoietin (hEPO). Also provided is an improved method for purifying rhEPO from culture medium by two-step column chromatography.