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METHOD FOR PRODUCING VIRUS-LIKE PARTICLES ON THE BASIS OF POLYOMA VIRUSES
METHOD FOR PRODUCING VIRUS-LIKE PARTICLES ON THE BASIS OF POLYOMA VIRUSES
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机译:一种基于多粒瘤病毒的类病毒颗粒的制备方法
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摘要
The invention relates to a method for producing virus-like particles on the basis of polyoma viruses. The invention can be used in medical fields such as gene therapy, vaccine development and diagnosis as well as in veterinary medicine. The aim of the invention is to develop a method for producing virus-like particles for gene therapy, diagnosis and vaccine development. This method should enable a highly efficient production of non-infectious, endotoxin-free virus-like particles which are suitable for humane applications. The inventive method relates to the application of a yeast high-expression system which permits the production of large quantities of virus-like particles on the basis of the main capsid protein VP1 of polyoma viruses. By extracting said particles from yeast cells, these virus-like particles are endotoxin-free and suitable for humane applications.
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