NOVEL NONVIRAL GENE CARRIER FOR TARGETING LIVER CANCER CELL AND PREPARATION THEREOF

摘要

PURPOSE: A method of delivering genes into liver cancer cells is provided by using ASOR (asialoorosomucoid), a gene carrier, which binds to ASGPr (asialoglycoprotein receptor) uniquely existing in a liver cancer cell. CONSTITUTION: The method comprises the following steps of: producing ASOR-PL conjugate by conjugating polylysine with ASOR; obtaining ASOR-PL-pCMV beta-gal plasmid complex, ASOR-PL-pSV2CAT plasmid complex or ASOR-PL-pCMV-p53 plasmid complex by linking plasmid pCMV beta-gal, pSV2CAT, pCMV-p53, respectively; treating the complex with polyoxyethylene-polyoxypropylene block copolymer before transfecting into liver cancer cell line.