NEW RAS-LIKE PROTEIN INTERACTING WITH SPECIFICALLY I KAPPA B, INHIBITOR OF NF KAPPA B

摘要

PURPOSE: New Ras-like protein which interacts with I kappa B, an inhibitor of NF kappa B, its gene and a separation method thereof from mice are provided which can be used for an anti-inflammatory agent, an immunomodulator, and an anticancer drug. CONSTITUTION: New Ras-like protein which interacts with I kappa B, an inhibitor of NF kappa B is separated from mice by using a yeast 2 hybrid method, determining its gene base sequence and amino acid sequence and a characteristic inhibiting NF kappa B activation intermediated by TNF alpha or IL-1 is examined. New Ras-like protein can be used for examining and searching a mechanism of NF kappa B or another signal transduction pathway. And this new Ras-like protein can be used for an anti-inflammatory agent, an immunomodulator, an anticancer drug, and its search system. The identification of Ras-like protein interacting with I kappa B by a glutathione S-transferase pull Down assay is shown in graph 2b.