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ALA(DELTHA AMINOLEVULINIC ACID) SYNTHASE-EXPRESSION ESHERICHIA COLI AND MANUFACTURING METHOD OF BIOSYNTHETIC DELTA-AMINOLEVULINIC ACID USING THE SAME
ALA(DELTHA AMINOLEVULINIC ACID) SYNTHASE-EXPRESSION ESHERICHIA COLI AND MANUFACTURING METHOD OF BIOSYNTHETIC DELTA-AMINOLEVULINIC ACID USING THE SAME
PURPOSE: ALA synthase-expression Escherichia coli is transformed with the expression vector. And a method for manufacturing biosynthetic ALA using the same is also provided CONSTITUTION: Provided is a method for producing ALA(biosynthesizing delta-aminolevulinic acid) using Escherichia coli which is in particular, transformed with an expression vector inserted hemA gene encoding ALA synthease of Bradyrhizobium japonicum, one of ALA synthesizing strain. The transformed Escherichia coli is cultivated in culture solution containing 30-120mM of glycine and 30-150mM of succinate and ALA is obtained by adding 30-90mM of levulinic acid thereto.
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