BETA-GLUCOSIDASE GENE ISOLATED FROM RUMINOCOCCUS ALBUS

摘要

PURPOSE: A beta-glucosidase gene sequence to be isolated from Ruminococcus albums is identified, and the expression vector inserting such gene and transformants thereby that are able to grow on the selective medium are obtained by using the genetic engineering techniques to produce a large amount of beta-glucosidase being useful in degrading cellulose. CONSTITUTION: A chromosomal DNA of Ruminococcus albums is cleaved with several restriction enzymes, so that its DNA library is prepared. The interest DNA fragments within the DNA library are inserted into a vector plasmid pUC 19. The gene is cloned through incubation of E. coli transformed by the pUC 19 vector. E. coli transformants by the pUC 19 vector containing the beta-glucosidase gene are isolated, which is determined by blue-colored colonization. After incubation of such isolated vector, the beta-glucosidase activity within the culture is measured.