Beta-glucosidase gene derived from Luminococcus albus

摘要

The present invention is to determine the nucleotide sequence of the beta-glucosidase gene derived from Ruminococcus albus (beta) using a genetic engineering technique to culture transformants transformed with the expression vector constructed by introducing the same A method for mass production of a glucosidase enzyme, wherein the chromosomal DNA of Luminococcus albus is digested with a restriction enzyme to prepare a DNA library, and a DNA fragment of the appropriate size obtained from the DNA library is inserted into a pUC19 vector. E. coli transformed with the vector and cultured to clone the beta-glucosidase gene, and the E. coli transformants carrying the cloned beta-glucosidase gene were selected and cultured. Beta-glucose of Luminococcus albus by measuring beta-glucosidase enzyme expression by measuring enzyme activity By the transformed E. coli culture it is introduced gene beta-an excellent effect of mass production of a glucosidase enzyme.