METHOD FOR PRODUCING SUBSTITUTE OF BONE USING ANIMAL BONES AND METHOD FOR SOLIDIFYING BONES

摘要

PURPOSE: A making method of substitute using animal bones is provided, which can replace damaged bone part of the human body into the new substitute without immune rejection, also reinforces the bones to use as a bone pin, a bone screw and a bone fixing plate. CONSTITUTION: A bone substitute producing method includes the steps of collecting bones by size and part from young healthy animal having no disease after examinations and cleaning, treating the bones in hydrogen peroxide vapor for over 15minutes, in 100% of alcohol or solution of mixing chloroform and methanol more than 24hours, in 0.6N of hydrochloric acid for 6-12hours according to needed parts and situations, treating the bones with Phosphate buffer solution over 30minutes, keeping the bones at -70C, performing lyophilization more than 24hours at a minimum, sterilizing by using electric beam, EO gas or 25KGy of irradiation, and immersing the bones resulted in a solution with antibiotics before use. Treating bones in hydrogen peroxide for 15minutes and processing lyophilization more than 24hours are the ways of solidifying the bones.