Expression of agfA of Salmonella enteritidis as a MBP-fused protein in Escherichia coli

摘要

PURPOSE: Provided is MBP-agfA fused protein to mass-expressed agfA, which is Salmonella enteritidis fimbriae protein, in E.coli. CONSTITUTION: Samonella enteritidis is composed SEF 21, 17 and 14 fimbriae proteins, in which SEF17 consisting of agfA has an excellent antigen property and a potentiality to be used as vaccine against infection of Salmonella. The agfA subunit of Salmonella enteritidis fimbriae itself is not water-soluble, thereby it is not easy to apply to vaccine. The agfA subunit is fused with maltose binding protein in order to be soluble in water. The agfA subunit fused with maltose binding protein is mass-expressed in E. coli.