CULTURE MEDIUM AUTO-RECYLING BIOREACTOR FOR PLANT TISSUE OR ORGAN CULTIVATION

摘要

The present invention relates to a medium-automatic bioreactor for plant tissue and organ culture, which is intended to obtain large amounts of cuttings or seeds by automatically circulating a liquid nutrient medium in a medium reservoir in culturing a large amount of plant tissues and organs. The automatic bioreactor of the present invention enables the production of biomass in large quantities in that it uses a liquid medium as compared to the conventional tissue culture method. In other words, by continuously circulating the liquid medium to supply the appropriate moisture and nutrient state to the cells in culture, it is possible to prevent vitrification of the culture and to easily set the production schedule such as injecting the medium suitable for the purpose of culture. In the case of a commonly used pump (palistaltic pump) can cause pollution, but in the present invention by the rational design, such as a simple electric circuit and the supply of periodic air pressure to prevent the contamination by suggesting a medium circulation device by the air pressure and gravity. In addition, the present invention was to maximize the photosynthetic efficiency of the culture by using a fluorescent lamp.