METHOD FOR PREPARING CULTURE MEDIUM FOR CULTURING PLANT TISSUE, METHOD FOR CULTURING PLANT TISSUE, STERILIZING AGENT, MICROBICIDAL AGENT, AND CULTURE MEDIUM COMPOSITION FOR CULTURING PLANT TISSUE

摘要

A culture medium for culturing a plant tissue includes: boiling a culture medium; adding a first sterilizing agent composed of a plurality of kinds of powdery agents to the culture medium; adding a second sterilizing agent composed of a single kind of agent to the culture medium; dispensing the culture medium into a culture vessel; cooling the dispensed culture medium; making the cooled culture medium and a plant tissue wet with a plurality of kinds of liquid agents; and introducing the plant tissue in the culture medium. By this invention, a method for preparing a culture medium for culturing a plant tissue and a method for inoculating a plant tissue can be provided in which the sterilizing or microbicidal treatment can be easily performed and yet the plant tissue can be grown to approximately the same extent as in the case of using an autoclave and a clean bench.