Culture method and sterilizing agent manufacturing method and plant tissue culture medium for plant tissue, sterilized agents, and plant tissue culture medium composition

摘要

A method for producing a culture medium for plant tissue of the present invention comprises a step of boiling a medium, a step of adding a first sterilization treatment agent composed of plural kinds of powdered medicines to the medium during boiling, a step of adding A step of adding a second sterilization treatment agent consisting of one type of drug to the culture medium and dispensing the culture medium into a culture container, cooling the dispensed medium, cooling the medium and the plant tissue in plural kinds A step of wetting a liquid medicine and a step of placing a plant tissue in the medium are carried out in order. This makes it possible to sterilize and sterilize conveniently and also to provide a method for producing a culture medium for cultivating a plant tissue capable of growing a plant tissue to the same extent as in the case of using an autoclave or a clean bench, Can be provided.