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Method of improving expression of granulocyte colony stimulating factor by fed-batch culture that controls the specific growth rate of cells
Method of improving expression of granulocyte colony stimulating factor by fed-batch culture that controls the specific growth rate of cells
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机译:分批补料培养提高粒细胞集落刺激因子表达的方法,控制细胞的比生长速度
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摘要
PURPOSE: Provided is a method for enhancing the expression of granulocyte colony stimulating factor G-CSF by constant feeding fed-batch that controls cell specific growth rate. Therefore, cell yield and expression of G-CSF are considerably enhanced and a plasmid stably is maintained in E. coli by latter stage of fermentation. CONSTITUTION: A method for enhancing expression of granulocyte colony stimulating factor G-CSF by constant feeding fed-batch is composed of the following steps of: (a) cultivating a recombinant E.coli transformed with G-CSF expression vector using L-arabinose operon of Salmonella spp. in high concentration; (b) inducing the expression of G-CSF using L- arabinose; (c) culturing by constant feeding fed-batch with adding feeding medium thereto.
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