PULLULANASE PRODUCING KLEBSIELLA SP. AND PROCESS FOR PRODUCING MALTOSYL BETA-CYCLODEXTRIN

摘要

PURPOSE: A microorganism Klebsiella sp. producing pullulanase and a method for producing maltosyl beta-cyclodextrin using the same are provided, thereby the maltosyl beta-cyclodextrin can be effectively and cheaply produced. CONSTITUTION: The microorganism Klebsiella sp. 192 isolated from the soil can produce pullulanase which hydrolyzes the alpha-1,6-glucosidic bond of pullulan and converts into maltotriose. The method for producing maltosyl beta-cyclodextrin comprises the steps of: purifying pullulanase from Klebsiella sp. 192; reacting an enzyme solution containing the purified pullulanase, 500 to 700 mg/ml of maltose and 80 to 150 mg/ml of beta-cyclodextrin at an appropriate temperature of about 45 to 55 deg. C and pH 4.0 to 5.0; and separating the reacting materials by subjecting the solution to chromatography.