HYBRID CELL PRODUCING MONOCLONAL ANTIBODIES FOR ESCHERICHIA COLI 0157 AND MONOCLONAL ANTIBODIES PRODUCED BY THEM

摘要

Purpose: specifically binding to Escherichia coli E.The fused cell line that coli O157 produce a kind of monoclonal antibody, which is provided to, rapidly and definitely detects Escherichia coli E.The infection of coli O157. Construction: specifically binding to Escherichia coli E.The microorganism NVRIO157 (KCTC 0429BP) that coli O157 produce a kind of monoclonal antibody is generated by step: using Escherichia coli E.Coli O157 exempt a mouse: by injecting Escherichia coli E.The H7 of coli O157: into mouse H7 as an antigen; Produce the separating immune unit an of antibody; Immunocyte is melted, myeloma cell produces NVRIO157 (KCTC 0429BP). Specifically binding to Escherichia coli E.Monoclonal antibody MNVRIO157: H7 of coli O157 is generated by NVRIO157 (KCTC 0429BP). The characteristic of monoclonal antibody MNVRIO157 is analyzed by ELISA.