METHOD OF MODELLING MEDICINAL LEECH EFFECT ON STIMULATION OF NERVOUS FIVER GROWTH IN CULTURED TISSUE

摘要

FIELD: biology, experimental medicine, hydrotherapy. SUBSTANCE: method involves culturing chicken embryo spinal ganglion on collagen substrate in medium containing calf fetal serum, Eagle medium, Henx solution and insulin in combination with addition of lyophilized medicinal leech head part an aqueous extract as a stimulating agent at concentration 200-400 ng/ml obtained after 24 h exposition or at concentration 100-200 ng/ml after 3 days exposition. Method helps to explain the presence of some effects of live medicinal leech on recovery of functions of nervous-muscular structure at organic damage of human nervous system cells, for example, in infantile cerebral paralysis and myopathy. EFFECT: enhanced effectiveness of method. 4 dwg, 3 ex