首页>
外国专利>
ENZYMATIC HYDROLYSIS OF 4-METHYLTHIOBUTYRONITRILES
ENZYMATIC HYDROLYSIS OF 4-METHYLTHIOBUTYRONITRILES
展开▼
机译:4-甲基硫代丁腈的酶促水解
展开▼
页面导航
摘要
著录项
相似文献
摘要
FIELD: organic chemistry, biochemistry. SUBSTANCE: method of enzymatic hydrolysis of 4-methylthiobutyronitriles to racemic 4-methyl-thiobutanoic acids is performed using nitrilase from Alcaligenes faecalis ATCC-8750 or Rhodococcus sp. HT 29-7 FERM-3857, or Gordana terrae FERM BP-4535 cells. Process is carried out at pH 4-11 and 30-60 C. Concentration α-substituted 4-methylthiobutyronitrile in the parent solution as substrate is 10-400 mmole/l, preferably 50-200 mmole/l. Hydrolysis process occurs without any selectivity with respect to enantiomers formed. EFFECT: improved method of hydrolysis. 6 cl, 1 dwg, 2 tbl, 16 ex
展开▼
机译:领域:有机化学,生物化学。物质:使用粪便产碱杆菌ATCC-8750或红球菌属的腈水解酶将4-甲基硫代丁腈酶解为外消旋的4-甲基-硫代丁酸的方法。 HT 29-7 FERM-3857或Gordana terrae FERM BP-4535细胞。方法在pH 4-11和30-60℃下进行。作为底物的母液中α-取代的4-甲基硫代丁腈的浓度为10-400mmol / l,优选50-200mmol / l。相对于所形成的对映异构体,水解过程没有任何选择性。效果:改进的水解方法。 6厘升,1载重吨,2汤匙,16前
展开▼