5 ID 50/ml. Viruses are concentrated by ultrafiltration by 10-20-fold, lipid envelope is separated by treatment with ether in cold and the end product that is peptide (molecular mass is 24-26 kDa) carrying group-specific antigen determinants is obtained. After the concentration stage viral material can be subjected for purification by high-speed centrifugation and ultracentrifugation additionally. After separation of lipid envelope obtained antigen is purified by affinity chromatography additionally. In this case the end product is immunoelectrophoretically pure peptide. Obtained cultural antigen is used for kit making for identification of antigen of equine infectious anemia virus. Kits for identification of antibodies are designated for diagnosis of equine infectious anemia in RDP or IFA. Kit for identification of antigen is designated for titration of antigen of equine infectious anemia virus in the process of culturing. Kit for identification of antibodies in RDP has cultural antigen and specific precipitating serum. Kit for identification of antibodies in IFA has cultural antigen for immobilization on solid carrier, polystyrene plate and antispecies immunoglobulin class G (Ig G) conjugated with horse radish peroxidase. Kit for identification of antigen has cultural antigen for immobilization on solid carrier, polystyrene plate and Ig G to equine infectious anemia virus conjugated with horse radish peroxidase. Kits for IFA have gelatin, substrate for peroxidase reaction carrying out, detergent and buffer solutions also. Kits can contain positive and negative controls also. EFFECT: enhanced sensitivity, standard specification and specificity of cultural antigen and kits for identification of antibodies and antigen. 18 cl, 6 ex"/>
METHOD OF PREPARING CULTURAL ANTIGEN FROM EQUINE INFECTIOUS ANEMIA VIRUS AND KIT FOR ANTIBODY OR ANTIGEN OF EQUINE INFECTIOUS ANEMIA VIRUS
FIELD: biotechnology, veterinary virology. SUBSTANCE: method involves the use of the novel strain of equine infectious anemia virus N "3-K-VNIITIBP-VIEV" for antigen preparing. Strain is grown in horse embryo skin and/or muscle, or lung, and/or kidney cells up to accumulation of viruses at titer 105 ID 50/ml. Viruses are concentrated by ultrafiltration by 10-20-fold, lipid envelope is separated by treatment with ether in cold and the end product that is peptide (molecular mass is 24-26 kDa) carrying group-specific antigen determinants is obtained. After the concentration stage viral material can be subjected for purification by high-speed centrifugation and ultracentrifugation additionally. After separation of lipid envelope obtained antigen is purified by affinity chromatography additionally. In this case the end product is immunoelectrophoretically pure peptide. Obtained cultural antigen is used for kit making for identification of antigen of equine infectious anemia virus. Kits for identification of antibodies are designated for diagnosis of equine infectious anemia in RDP or IFA. Kit for identification of antigen is designated for titration of antigen of equine infectious anemia virus in the process of culturing. Kit for identification of antibodies in RDP has cultural antigen and specific precipitating serum. Kit for identification of antibodies in IFA has cultural antigen for immobilization on solid carrier, polystyrene plate and antispecies immunoglobulin class G (Ig G) conjugated with horse radish peroxidase. Kit for identification of antigen has cultural antigen for immobilization on solid carrier, polystyrene plate and Ig G to equine infectious anemia virus conjugated with horse radish peroxidase. Kits for IFA have gelatin, substrate for peroxidase reaction carrying out, detergent and buffer solutions also. Kits can contain positive and negative controls also. EFFECT: enhanced sensitivity, standard specification and specificity of cultural antigen and kits for identification of antibodies and antigen. 18 cl, 6 ex
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