METHOD OF DETECTION OF CATTLE VIRAL DIARRHEA VIRUS (MUCOSA DISEASE) USING SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION

摘要

FIELD: molecular biology, veterinary science. SUBSTANCE: invention relates to method that can be used for detection of cattle viral diarrhea. Method involves synthesis of oligonucleotide primers 5'-TAG-ATT-CCA-GAC-CGA-CCC-3' and 5'-GGA-AGG-GAT-ACA-ACG-GGC-3' and synthesis of complementary DNA on DNA matrix. Then amplification of synthesized DNA is carried out by method of polymerase chain reaction and polymerase chain reaction products size is analyzed. Proposed method ensures to differentiate cattle viral diarrhea virus from the close-related classical porcine plague virus. EFFECT: improved method of virus detection. 1 tbl, 1 ex