Process for the modification and identification of functional sites in proteins

摘要

The invention relates to a method for identifying one or more functional sites in a protein, which is characterized in that DOLLAR A a) the target protein is brought into contact with a binding partner (BP tag) linked to a laser-activatable marker (tag), wherein a complex of target protein and BP tag is formed, DOLLAR A b) the complex of target protein and BP tag is irradiated with laser light, thereby generating free radicals which selectively change the bound target protein at the binding site, and DOLLAR A c) selectively altered region of the protein identified by a combination of protein cleavage and mass spectrometry. The invention further relates to a device for carrying out the method according to the invention.