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A method for the preparation of a monoclonal antibody having specificity for crosslinked fibrin derivatives and a test method by means of this antibody.
A method for the preparation of a monoclonal antibody having specificity for crosslinked fibrin derivatives and a test method by means of this antibody.
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机译:制备对交联的血纤蛋白衍生物具有特异性的单克隆抗体的方法和利用该抗体的测试方法。
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摘要
The invention relates to a method of a preparation of monoclonal antibody derived from a crosslinked fibrin derivative including the steps of: (i) obtaining a crosslinked fibrin derivative or extract containing same; and (ii) forming an antibody to said derivative or extract by cloning antibody producing cells from an animal having been administered thereto said derivative or extract. A screening assay for use in the abovementioned method which includes the steps of: (A) coating a surface with an antigen selected from a (i) crosslinked fibrin derivative; (ii) extract containing said derivative; or (iii) fibrinogen degradation product. (B) contacting said antigen with a monoclonal antibody derived from crosslinked fibrin derivative; and (C) subjecting the complex formed in step (A) to a signal amplification step and an assay procedure for detection of the presence of a crosslinked fibrin derivative in an animal body fluid including the steps of: (i) contacting a monoclonal antibody prepared from a crosslinked fibrin derivative with a fluid sample suspected of containing an antigen derived from a crosslinked fibrin derivative or comprising a crosslinked fibrin derivative per se; and (ii) subjecting the complex formed in step (i) to a signal amplification step are also provided.
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