method for the determination of antibodies in seronegative individuals

摘要

The present invention relates to an improved method and kit for the detection of antibodies directed against the human immunodeficiency virus (HIV) in whole blood obtained from individuals who are HIV-seronegative as determined by conventional assay techniques. Whole blood is isolated from these individuals and cultured directly with mitogen to stimulate the proliferation of B-lymphocytes producing immunoglobulin specific for HIV. This method provides a rapid, simple, and inexpensive means for screening large numbers of blood samples for HIV infection while minimizing exposure of the technician to infectious blood components.