Rapid assays for the determination of the member state based on the detection of one or more isoenzymes of glutathione - s - transferase

摘要

A method for the rapid detection of the various isoenzymes of glutathione S-transferase (GST) in diverse biological fluids comprises contacting a sample of the biological fluid with an anti-GST antibody bound to a solid phase and determining the presence of bound GST in one or more steps. The biological fluid which can be, for example, bile, plasma, serum or urine is preferably contacted with particle-labelled anti-GST antibody, so that the GST isoenzyme to be detected is bound to said particle-labelled anti-GST antibody prior to being contacted with solid phase immobilised antibody. The anti-GST antibody used has a sensitivity sufficient to detect picomolar or lower amounts of GST isoenzymes. The method permits the detection or determination of GST isoenzymes typically in a time of the order of 30 minutes from initial contact with solid phase anti-GST. The method is simple to use and thus can be used, for example, by patients at home to monitor their levels of a particular GST isoenzyme following a liver or renal transplant.