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Method for cloning and producing the NspI restriction endonuclease in E. coli and purification of the recombinant NspI restriction endonuclease
Method for cloning and producing the NspI restriction endonuclease in E. coli and purification of the recombinant NspI restriction endonuclease
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机译：在大肠杆菌中克隆和生产NspI限制性核酸内切酶的方法以及重组NspI限制性核酸内切酶的纯化
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摘要
The present invention relates to clones which express the recombinant NspI restriction endonuclease using a NspI methylase premodified E. coli K strain RR1 (&lgr;DE3) and to methods for producing and purifying said enzyme.
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