Membrane filter agar medium containing two enzyme substrates used for the simultaneous detection of total coliforms and E. coli.

摘要

An improved method for detection of total coliforms and E. coli comprising placing the target sample in a broth containing an ingredient that will encourage growth and repair of injured coliforms, at least one agent that suppresses growth of gram positive cocci and spore- forming organisms, at least one active agent that will suppress growth of non- coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. In the preferred embodiment, both a fluorogen and chromogen were used. Preferred methods include use of filter and/or plates containing the growth- promoting ingredients and the indicators.