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HBV polymerase, RNase H enzyme derived from HBV polymerase, processes for preparation and uses for screening antiviral agents thereof
HBV polymerase, RNase H enzyme derived from HBV polymerase, processes for preparation and uses for screening antiviral agents thereof
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机译:HBV聚合酶,源自HBV聚合酶的RNase H酶,其制备方法及其筛选抗病毒剂的用途
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摘要
The present invention relates to hepatitis B virus (hereinafter it refers to HBV) polymerase containing a histidine tag, RNase H enzyme derived from HBV polymerase and processes for preparation thereof. More particularly, the present invention relates to recombinant HBV polymerase, its RNase H domain with enzyme activity, expression vectors producing the enzymes in E. coli and processes for preparing the HBV polymerase and the RNase H enzyme which can be easily purified due to their histidine tags. And the present invention relates to uses of the HBV polymerase and the RNase H enzyme for screening antiviral agents.
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