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Agents and a method of chromosome preparation using protein phosphatase inhibitors induced premature chromosome condensation (PCC) technique
Agents and a method of chromosome preparation using protein phosphatase inhibitors induced premature chromosome condensation (PCC) technique
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机译:使用蛋白磷酸酶抑制剂诱导染色体过早凝结(PCC)技术的药物和染色体制备方法
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摘要
Agents and a method for generating chromosomes by premature chromosome condensation (PCC) technique utilizes inhibitors of serine/threonine protein phosphatases. When the cells were treated with these agents, they underwent PCC at any phase of cell cycle within 2 hours. This method enables to obtain not only chromosome of mitotic cells but also those from interphase nuclei much easily, quickly and effectively.
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