HPLC apparatus for preparative sampling and preparation of NMR samples and method for converting mobile phase

摘要

Problem to be solved: to provide a high-performance liquid chromatography apparatus capable of efficiently separating and preparing trace samples for NMR measurement and a method of converting a mobile phase. The components of the sample were separated by high performance liquid chromatography, and the component was captured by the trapping column, and the water was replaced by the heavy water, and the following components were eluted from the trapping column by the heavy solvent other than heavy water. .