E1 protein gene of variant pyruvic acid dehydrogenase complex and E1 protein null of variant pyruvic acid dehydrogenase

摘要

PURPOSE: To provide a new gene useful for the production of E₁ protein of a variant pyruvic acid dehydrogenase complex. ;CONSTITUTION: The E₁ protein gene of a variant pyruvic acid dehydrogenase complex has a function to code an amino acid sequence having a structure obtained by substituting the 146th arginine in the amino acid sequence of E₁ protein of wild-type pyruvic acid dehydrogenase complex with proline. The objective gene has the base sequence of formula and is obtained form plasmid pACEE1K' DNA separated from E.coli JM109 (ferm BP-4486).;COPYRIGHT: (C)1994,JPO