(57) to support the growth of embryonic stem cells in culture, [summary] The present invention provides a serum-free supplement. There is also provided a medium containing a basal medium supplemented with serum-free supplement of the present invention. The present invention provides a transgenic animal and embryonic stem cells, the present invention provides methods for expressing recombinant proteins and methods for producing methods, transgenic animals for isolating and culturing the embryonic stem cells .
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