The trypsin way production manner null of nuclear acid base array and the said enzyme which the enzyme the code

摘要

PURPOSE: To obtain the subject new base sequence coding a trypsin-like enzyme, etc., having a specific amino acid sequence, selectively decomposing fibrinogen, etc., and existing in the respiratory tract of a human lung, and giving the enzyme useful for treating circulatory diseases, etc., as an anticoagulant. CONSTITUTION: The new nucleic acid base sequence codes a new trypsin-like enzyme or its biochemical equivalent having an amino acid sequence containing an amino acid sequence of the formula, existing in the respiratory tract of a human lung, selectively decomposing trypsin and thrombin-synthesizing substrate and fibrinogen, having an action for prolonging a blood-coagulating time, and useful for treating circulatory diseases such as chronic arterial obstruction syndrome and peripheral circulatory disorders. The nucleic acid base sequence is obtained by synthesizing two oligonucleotides composing a part of a gene coding the amino acid sequence of a trypsin-like enzyme separated from the sputum of a chronic respiratory organ disease patient from the gene, and subsequently cloning a human respiratory tract cDNA in the presence of the synthesized oligonucleotides as primers by a PCR method.