NITRILE HYDRATASE GENE AND AMIDASE GENE DERIVED FROM BACTERIUM BELONGING TO THE GENUS RHODOCOCCUS

摘要

PROBLEM TO BE SOLVED: To obtain a DNA sequence of a gene coding for nitrile hydratase which is a nitrile-decomposing enzyme derived from bacteria belonging to the genus Rhodococcus and exhibiting especially excellent position selectivity to nitriles and especially to a nitrile group in an aromatic polynitrile compound and a gene coding for amidase and to provide a method for producing an enzyme protein using the DNA sequence and further a method for producing amides and carboxylic acids using the enzyme. ;SOLUTION: This nitrile hydratase gene is composed of the DNA sequence coding for a specific amino acid sequence and derived from the bacteria belonging to the genus Rhodococcus and the amidase gene is composed of the DNA sequence coding for a specified amino acid sequence. The method for producing the amides and the carboxylic acids comprises transforming the nitrile group in the nitriles to an amide group and further a carboxy group by using a transformant transformed by a plasmid containing the nitrile hydratase and/or amidase gene DNA.;COPYRIGHT: (C)2001,JPO