IMMUNOASSAY, METHOD FOR ELIMINATING IMMUNOREACTIVE INTERFERENCE SUBSTANCE, AND REAGENT FOR MEASURING IT

摘要

PROBLEM TO BE SOLVED: To sufficiently suppress interference operation even if an interference sub stance is contained in a specimen to be measured with high concentration by performing an antigen/antibody reaction under the presence of low-class oxygen acid salt and/or a transition metal. ;SOLUTION: A low-class oxygen acid salt is selected from sulfite, hyposulfite, phosphite, and the like for use, and especially sodium sulfite is preferable and approximately 1-1000 mM concentration is contained. At concentration that is less than 1-1000 mM, no interference substance for immunoreaction can be suppressed. On the other hand, at concentration that is equal to or more than 1-1000 mM, solution viscosity becomes high, thus poorly affecting a measurement value. A transition metal is selected from copper, nickel, cobalt, and the like, especially nickel acetate is preferable and the transition metal is contained by approximately 0.1-100 mM. At a concentration that is less than 0.1-100 mM, rheumatism factors and heterophil antibodies cannot be suppressed. On the other hand, at a concentration that is equal to or more than 0.1-100 mM, a measurement value is poorly affected. By suppressing the protein protection agent of inactive protein such as albumin and gelatin as needed, the stabilization effect of a substance to be measured can be improved.;COPYRIGHT: (C)2001,JPO