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Method of producing an enzyme which undergoes cleavage in the secretory glycosyltransferases and other Golgi apparatus
Method of producing an enzyme which undergoes cleavage in the secretory glycosyltransferases and other Golgi apparatus
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机译:产生在分泌型糖基转移酶中裂解的酶的方法和其他高尔基体
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摘要
A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.
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