Plastid Transformation method of improved Nucleic Acid Molecule to be used in said Method, Chimeric Gene, vector transformation and deplanta, Plant Cell, PlantPlant Seed and Plant Tissue, or plastid transgenic Plant.

摘要

Promoter isolated from the 5 flanking region upstream of the coding sequence of the plu00e1stido ClpP Gene of Arabidopsis.Use method of Protein Coding regions of genes to isolate plu00e1stido Regulatory sequences involved.And a method to improve the efficiency of transformation of plu00e1stido plu00e1stido exogenous Promoters that differ in Nucleotide sequence of the promoters of plu00e1stidos natives.Improved Method of plu00e1stido DETRANSFORMACION, comprising a plu00e1stido transform a kind of host PlantA Chimeric Gene comprising a secuenciareguladora active in plu00e1stido operatively linked to a Coding sequence of interest.Where the regulatory sequence denucleotidos has a sequence that is less than about 90% identical to a regulatory sequence in the corresponding Native Plant huu00e9spe plu00e1stidoD, which reduces the undesired somatic recombination between the regulatory sequence in the Chimeric Gene and Regulatory sequence in the corresponding elplu00e1stido Native host Plant.Nucleic Acid Molecule to be used in said Method, Chimeric Gene, Vector and Transformation of Plant, Plant, Plant Cell, Plant SEED, Plant Tissue,Or plu00e1stido transgenic Plant, which comprises each Chimeric Gene.