Isolation process of physalins from plants and pharmaceutical compositions with anti-protozoa activity containing physalins

摘要

'' PHYSALINE INSULATION PROCESS FROM PLANTS AND PHARMACEUTICAL COMPOSITIONS WITH ANTI-PROTOZOA ACTIVITY CONTAINING PHYSALINES ''. The present invention relates to the use of pharmaceutical compositions containing extracts or fractions of Physalis angulata in the treatment of diseases caused by protozoa. Another embodiment of the present invention is the process of isolating physalins from plants of the Solonaceae family characterized by containing the following steps: (a) grinding the roots, leaves or stems of Physalis spp .; (b) extracting said roots, leaves or stems from step (a) with an alcoholic solvent, cold or hot, for an appropriate period of time; (c) evaporating the organic extract from step (b) and washing the syrupy material with a non-polar organic solvent; (d) purification of the extract subjected to washing step (c) by rapid filtration in a silica gel H adsorbent, followed by a polarity gradient with organic solvents; and (e) steroid separation using chromatography.