System for use in the production of a polyketide, multi-enzyme pks, nucleic acid, vector, transforming organism, variant of a parental polyketide and process for preparing a polyketide.

摘要

B '' SYSTEM FOR USE IN THE PRODUCTION OF A POLYCETIDE, MULTI-ENZYME PKS, NUCLEIC ACID, VECTOR, TRANSFORMING ORGANISM, VARIANT TO A PARENTAL POLYCETIDE AND PROCESS TO PREPARE A POLYCETIDE '' D A polyketide ' ") type I is a complex multi-enzyme including a load domain linked to a multiplicity of extension domains. The first extension module receives an acyl initiator unit from the load domain and each extension module adds another unit of extension. cetid that can undergo processing (for example reduction) .The applicants found that the Ksq domain owned by some of the PKS's has decarboxylating activity, for example generating acyl (substituted) from malonyl (substituted). The CLF domain of type II PKS's has similar activity. By inserting load modules including these domains in PKS's not normally having them, it is possible to control the starter units used.