Method for production of a culture of Secretariat processed, variedadde, maize starch isolated from the seed of a variety of maize, flour prepared from a variety of culture of cereal.Preparation method of a compound food thickened, variety of maize transformed with a chimeric gene and starch.

摘要

b "method of production of a culture of cereal processing, a variety of maize, starch isolated from the kernels of a variety of maize, flour prepared from a variety of culture of cereal.Preparation method of a compound food thickened.The variety of maize transformed with a chimeric gene and starch " d this invention presents the use of a clone of cDNA to construct genes with sense and anti sense to the inhibition of the ITAVity starch synthase enzyme in maize.More specifically, the present invention relates to a method of controlling the fine structure of the starch derivative of grain from cereal crops.Which comprises: (1) the preparation of a chimeric gene comprising a fragment of a nucleic acid encoding a starch synthase structural gene or a fragment of it.Operationally connected in orientation with a sense or anti sense on the anterior side, a fragment of a nucleic acid encoding a promoter that directs the expression of the gene in endosperm tissueMaize,And operationally connected at the rear side of a fragment of a nucleic acid sequence encoding a regulator suitable for termination of transcription; and (2) the transformation of cultures of EcREAIS for the said chimeric gene in which the expression of the chimeric gene results in the change of said fine structure of starch derivative of grain of these cereal crops processed.In comparison with the fine structure of starch derived from cereal crops that do not possess the said chimeric gene.