DETECTION OF MUTUATIONS ON ENDOTHELIAL NITROGEN MONOXIDE SYNTHASE (eNOS) GENE FOR DIAGNOSIS AND SCREENING OF CORONARISM

摘要

A process for screening for a gene involving a coronary artery spasm- associated disease, which comprises detecting the presence of one or more nucleotide changes in the gene of endothelial nitric oxide synthase (eNOS), which changes are selected from a group consisting of the changes from guanine (G) to thymine (T) at position 894, and from cytosine (C) to thymine (T) at position 774 of the cDNA sequence of the eNOS gene, and the changes from thymine (T) to cytosine (C) at position -786, from adenine (A) to guanine (G) at position -922, and from thymine (T) to adenine (A) at position - 1468 of the 5'-flanking region of the eNOS gene, as well as the changes at the corresponding positions in the complementary strand thereof.