Process and kit for permeabilizing erythrocytes, and immuno-marking foetal erythrocytes

摘要

Provided is a process for permabilising erythrocytes in vitro characterised in that the erythrocytes are subjected successively to the action of a)a fixing agent containing an aliphatic aldehyde, an oligosaccharide and optionally a sulfated poysaccharide in preferably an isotonic or hypertonic medium with a pH of between 4 and 7; and b)a permeabilising agent containing a detergent and an oligosaccharide preferably with a pH of 5 to 6; and optionally c)after fixation and permeabilisation, the erythrocytes are washed using a washing agent to eliminate the detergent; and optionally the erythrocytes are reacted with two antibodies against two foetal antigens which are expressed independently on the erythrocyte. Also provide is a suitable kit for permeabilising erythrocytes comprising agents a) and b) above.