Micro Electro-permeabilization System for Cell Medium Conductivity Change Measurement of Erythrocytes Cells

摘要

The mechanism of electroporation is used in a wide range of medical applications, genetic engineering, and therapies. Measurements of biological cells suspension conductivity have been used to evaluate the effect of electroporation cells. A micro electro-permeabilization system was built to apply 900 us pulses with electric field variations in a cells suspension. The electrodes have distance around 200 micrometers. We observed changing in the cells suspension conductivity as the increased electric field with the micro electro-permeabilization system. During electroporation we conclude that different volume fractions leads different conductivities of cells suspension in same electric field, in low-conductive medium increasing the electric field induce a higher conductivity gradient of cells suspension in lower concentration of cells, and for same cells volume fractions and same electric field results different conductivity of cells suspensions changing external medium conductivity.