PROCESS FOR PREPARING MICROBIAL PROTEINS BY BIOTRANSFORMATION OF WASTE VEGETABLE MATTER

摘要

The invention relates to a process for preparing microbial proteins by biotransformation of the waste vegetable matter, consisting of the biosynthesis of the cellulolytic enzyme complex in a discontinuous submerged culture of a strain of Aspergillus niger in nutritive medium containing 1...2% crushed corn cobs and 2...3% soya bean groats at an acidic pH, followed by the enzyme preparation by settling, centrifuging or atomizing the fermented medium, the enzymatic hydrolysis of a mixture comprising 2% vegetable substrate pre-treated mechanically, thermally by drying at 90...100°C and chemically with 1...2% NaOH or H2SO4, the concentration of the added enzyme ranging between 50 and 100 UCx/g of substrate, the third stage consisting of the fermentation of hydrolysis products by inoculating the mixture with a pure or mixed culture of yeasts of the genera Candida and Saccharomyces, such as the species Candida robusta, Candida scotii, Saccharomyces diastaticus, S. cerevisiae, while maintaining the bioprocess for 24...48 hours at a temperature of 28...30°C, stirring at 200...400 rpm, aeration of 0,3...0.5 l/1 min and pressure of 0.3...0.5 at, the final stage consisting in conditioning the yeast biomass by centrifuging at 2,000...4,000 rpm for 20...30 min and drying at 100...105°C for 2...4 hours.