Promoters n-acetilhexosaminidas beta-and gamma-actin and its use in expression systems, and secretion of filamentous fungi antisense glutamate dehydrogenase genes.

摘要

Promoters of glutamate dehydrogenase genes, {b -N-acetylhexosaminidase and {y -actin and its use in expression systems, secretion and antisense filamentous fungi. glutamate dehydrogenase b -N-acetilhexosa-minidasa (EC.3.2.1.52) of (I) NADP dependent (EC.1.4.1.4) of Penicillium chrysogenum, (II) {: the use of promoters of genes encoding it described Penicillium chrysogenum and (III) and {-actin and Acremonium chrysogenum Penicillium chrysogenum, which can be used for the construction of potent vectors of expression and secretion useful both for P. chrysogenum and for A. chrysogenum and related species. Moreover, these promoters can be used for blocking gene expression by antisense constructs. Under control of the aforementioned promoters can drive expression of other genes in filamentous fungi, increasing the production of antibiotics and / or proteins inherent therein.