PROCESS FOR THE PREPARATION IN PURE FORM OF THE PROTEASE ACTIVATING BLOOD CLOTTING FACTOR VII, ITS PROENZYME OR A MIXTURE OF BOTH PROTEINS BY MEANS OF AFFINITY CHROMATOGRAPHY

摘要

A process for the preparation in pure form of the protease activating bloodclottingfactor VII and/or its proenzyme by use of one or more affinity chromatographyseparation processes and/or fractional precipitation is described, in whichthe affinitychromatography separation process used is adsorption on- calcium phosphate/hydroxyapatite,- a hydrophobic matrix,- a chelate matrix,- a matrix on which heparin or a substance related to heparin, such asheparan sulfate or dextran sulfate, is immobilized, and/or- a matrix which is coated with an immobilized monoclonal or polyclonalantibody directed against the protein to be isolated, or its F(ab) orF(ab)2 fragments.A pharmaceutical preparation and a reagent are moreover described whichcontainthe said protease and its proenzyme.