Process for the preparation in pure form of the protease activating blood clotting factor VII its proenzyme or a mixture of both proteins by means of affinity chromatography

摘要

PURPOSE: Provided are methods for purification of factor VII activating protease, its proenzyme or a mixture of both forms, by affinity chromatography, thereby treating symptoms of thrombosis complication. CONSTITUTION: The methods for purifying factor VII-activating protease and/or its precursor comprises using one or more: (a) affinity chromatography separations which involves adsorption on: (i) calcium phosphate/hydroxylapatite; (ii) a hydrophobic matrix; (iii) a chelating matrix; (iv) a matrix carrying heparin or a related substance; and/or (v) a matrix carrying antibodies or their Fab/Fab2 fragments specific for factor VII-activating protease; and/or (b) fractional precipitations.