AN ELECTROCHEMICAL METHOD FOR ENRICHMENT OF MICROORGANISM, A BIOSENSOR FOR ANALYZING ORGANIC SUBSTANCE AND BOD

摘要

Disclosed herein is a biosensor that allows an organic substance concentrationor BOD of a sample to be electrochemically measured in anaerobic conditionusing a mediator-less biofuel cell. The biosensor utilizes electrochemicallyactive bacteria that were contained in wastewater and sluge and denselycultured during the operation procedure of the biofuel cell for the BODmeasurement, as a microbial catalyst of the biofuel cell used in thebiosensor. As a result, the biosensor can be operated without an artificialaddition of microorganisms, and allows the microorganisms to be maintained ata suitable activity depending on the nature of wastewater. In addition, thebiofuel cell used in the biosensor can be operated in a stable manner over sixmonths or more.