SEROTYPE-SPECIFIC IDENTIFICATION OF ENTEROVIRUS 71 BY RT-PCR

摘要

The present invention provides nucleic acids which can be used as primers inamplification or sequencing reactions to rapidly amplify or sequence,respectively, EV71 nucleic acids. A preferred group of nucleic acids of thepresent invention when used as primer pairs in amplification reactions, detectEV71 with a high degree of specifically and sensitivity. With these preferredprimer pairs, the specificity of amplification methods of the presentinvention is such that EV71 nucleic acid is amplified to a detectable level,whereas CA16 DNA is not. The nucleic acid primers of the present inventioncontain mixed bases or deoxyinosine residues at positions of codon degeneracy.Examples of nucleic acid primers for amplifying and sequencing EV71 nucleicacid are set forth in the Sequence Listing as SEQ ID NOS:1-12. Examples ofpreferred primers for discriminating between EV71 and CA16 are set forth inthe Sequence Listing as SEQ ID NOS:1-4.